MiaA

• Description: tRNA isopentenylpyrophosphate transferase
  
  

Gene name	miaA
Synonyms
Essential	no
Product	tRNA isopentenylpyrophosphate transferase
Function	tRNA modification
Gene expression levels in SubtiExpress: miaA
MW, pI	35 kDa, 6.002
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	ymaF, hfq
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU17330

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)

• Protein family: IPP transferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31795

• KEGG entry: [2]

• E.C. number: 2.5.1.8

Expression and regulation

• Operon:

• Expression browser: miaA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

The corresponding gene/ protein in other bacteria

J Zhao, H E Leung, M E Winkler
The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.
J Bacteriol: 2001, 183(5);1796-800
[PubMed:11160115] [WorldCat.org] [DOI] (P p)

J M Durand, G R Björk, A Kuwae, M Yoshikawa, C Sasakawa
The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes.
J Bacteriol: 1997, 179(18);5777-82
[PubMed:9294434] [WorldCat.org] [DOI] (P p)

J Caillet, L Droogmans
Molecular cloning of the Escherichia coli miaA gene involved in the formation of delta 2-isopentenyl adenosine in tRNA.
J Bacteriol: 1988, 170(9);4147-52
[PubMed:3045085] [WorldCat.org] [DOI] (P p)