Difference between revisions of "PbuE"
| Line 1: | Line 1: | ||
| − | + | ||
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 13: | Line 13: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || nucleotide metabolism | |style="background:#ABCDEF;" align="center"|'''Function''' || nucleotide metabolism | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU05800 pbuE] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 8.884 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 8.884 | ||
Revision as of 15:30, 6 August 2012
| Gene name | pbuE |
| Synonyms | ydhL |
| Essential | no |
| Product | putative hypoxanthine exporter |
| Function | nucleotide metabolism |
| Gene expression levels in SubtiExpress: pbuE | |
| MW, pI | 41 kDa, 8.884 |
| Gene length, protein length | 1164 bp, 388 aa |
| Immediate neighbours | ydhK, gmuB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, nucleotide metabolism/ other, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU05800
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q797E3
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- A-box: adenine-controlled riboswitch, transcription termination/ antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jean-François Lemay, Guillaume Desnoyers, Simon Blouin, Benoit Heppell, Laurène Bastet, Patrick St-Pierre, Eric Massé, Daniel A Lafontaine
Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.
PLoS Genet: 2011, 7(1);e1001278
[PubMed:21283784]
[WorldCat.org]
[DOI]
(I e)
Vanessa Delfosse, Patricia Bouchard, Eric Bonneau, Pierre Dagenais, Jean-François Lemay, Daniel A Lafontaine, Pascale Legault
Riboswitch structure: an internal residue mimicking the purine ligand.
Nucleic Acids Res: 2010, 38(6);2057-68
[PubMed:20022916]
[WorldCat.org]
[DOI]
(I p)
Elias Seif, Sidney Altman
RNase P cleaves the adenine riboswitch and stabilizes pbuE mRNA in Bacillus subtilis.
RNA: 2008, 14(6);1237-43
[PubMed:18441052]
[WorldCat.org]
[DOI]
(I p)
Natalia P Zakataeva, Sergey V Gronskiy, Anastasia S Sheremet, Ekaterina A Kutukova, Anna E Novikova, Vitaliy A Livshits
A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides.
Res Microbiol: 2007, 158(8-9);659-65
[PubMed:17935948]
[WorldCat.org]
[DOI]
(P p)
K V Lobanov, N V Korol'kova, S Iu Eremina, L Errais Lopes, S A Proshkin, A S Mironov
[Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene].
Genetika: 2007, 43(6);859-64
[PubMed:17853814]
[WorldCat.org]
(P p)
Jean-François Lemay, J Carlos Penedo, Renaud Tremblay, David M J Lilley, Daniel A Lafontaine
Folding of the adenine riboswitch.
Chem Biol: 2006, 13(8);857-68
[PubMed:16931335]
[WorldCat.org]
[DOI]
(P p)
J Kenneth Wickiser, Ming T Cheah, Ronald R Breaker, Donald M Crothers
The kinetics of ligand binding by an adenine-sensing riboswitch.
Biochemistry: 2005, 44(40);13404-14
[PubMed:16201765]
[WorldCat.org]
[DOI]
(P p)
Per Nygaard, Hans H Saxild
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
J Bacteriol: 2005, 187(2);791-4
[PubMed:15629952]
[WorldCat.org]
[DOI]
(P p)
Maumita Mandal, Ronald R Breaker
Adenine riboswitches and gene activation by disruption of a transcription terminator.
Nat Struct Mol Biol: 2004, 11(1);29-35
[PubMed:14718920]
[WorldCat.org]
[DOI]
(P p)
Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093]
[WorldCat.org]
[DOI]
(P p)
Maumita Mandal, Benjamin Boese, Jeffrey E Barrick, Wade C Winkler, Ronald R Breaker
Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria.
Cell: 2003, 113(5);577-86
[PubMed:12787499]
[WorldCat.org]
[DOI]
(P p)
