Difference between revisions of "ThrS"

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* '''Regulation:'''  
 
* '''Regulation:'''  
 
** induced by threonine limitation ([[T-box]]) {{PubMed|19258532,8288542}}  
 
** induced by threonine limitation ([[T-box]]) {{PubMed|19258532,8288542}}  
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** expression transiently increases in the forespore {{PubMed|22848659}}
  
 
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* '''Regulatory mechanism:'''  
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=References=
 
=References=
  
<pubmed>8288542,1379177,19258532,7476165,12136084,8969504 ,17114254, </pubmed>
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<pubmed>8288542,1379177,19258532,7476165,12136084,8969504 ,17114254, 22848659</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:14, 1 August 2012

  • Description: threonyl-tRNA synthetase (major)

Gene name thrS
Synonyms
Essential no
Product threonyl-tRNA synthetase (major)
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 73 kDa, 5.214
Gene length, protein length 1929 bp, 643 aa
Immediate neighbours ysaA, ytxC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThrS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThrS expression.png



















Categories containing this gene/protein

translation

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28950

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s): ThrZ, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1TJE (from Escherichia coli, 44% identity, 63% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • induced by threonine limitation (T-box) PubMed
    • expression transiently increases in the forespore PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References