SsbB

• Description: single-strand DNA-binding protein
  
  

Gene name	ssbB
Synonyms	ywpH
Essential	no
Product	single-strand DNA-binding protein
Function	required for efficient genetic transformation
Interactions involving this protein in SubtInteract: SsbB
MW, pI	12 kDa, 6.876
Gene length, protein length	339 bp, 113 aa
Immediate neighbours	glcR, ywpG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA replication, DNA repair/ recombination, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU36310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds and protects internalized foreign ssDNA PubMed

• Protein family:

• Paralogous protein(s): SsbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-55 PubMed
  • phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed

• Cofactor(s):

• Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed

• Interactions:
  • forms tetramers PubMed
  • SsbB-ComFA PubMed
  • SsbB-CoiA PubMed
  • SsbB-DprA PubMed
  • SsbB-RecA PubMed

• Localization:
  • localizes to cell poles in competent cells PubMed
  • localizes to the DNA entry pole during transformation PubMed

Database entries

• Structure: 3VDY 3VDY (in complex with ssDNA) PubMed

• UniProt: C0SPB6

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ssbB PubMed
  • ssbB-glcR PubMed (this is unlikely according to PubMed)

• Expression browser: ssbB PubMed

• Sigma factor:

• Regulation:
  • expressed at the onset of stationary phase PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: