Hbs

• Description: non-specific DNA-binding protein Hbsu
  
  

Gene name	hbs
Synonyms	dbpA, hupA
Essential	yes PubMed
Product	non-specific DNA-binding protein Hbsu
Function	DNA packaging, function of the signal recognition complex
MW, pI	9 kDa, 9.501
Gene length, protein length	276 bp, 92 aa
Immediate neighbours	folE, spoIVA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

LexA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU22790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Hbs-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed

• Protein family: DbpA subfamily (according to Swiss-Prot)

• Paralogous protein(s): YonN

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-61 PubMed
  • phosphorylation on Thr-4 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • associated with the nucleoid in vegetative cells and forespores PubMed
  • membrane associated PubMed

Database entries

• Structure: 1HUU (Geobacillus stearothermophilus)

• UniProt: P08821

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hbs PubMed

• Expression browser: hbs PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:
  • mRNA is protected from degradation by RnjA by initiating ribosomes PubMed
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Ciaran Condon, IBPC, Paris, France Homepage
• Mohamed Marahiel, University of Marburg, Germany Homepage

References

Reviews

Wolfgang Klein, Mohamed A Marahiel
Structure-function relationship and regulation of two Bacillus subtilis DNA-binding proteins, HBsu and AbrB.
J Mol Microbiol Biotechnol: 2002, 4(3);323-9
[PubMed:11931565] [WorldCat.org] (P p)