Difference between revisions of "SacT"
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* '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}} | * '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}} | ||
| − | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}} |
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| + | * '''Sigma factor:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 08:58, 17 April 2012
- Description: transcriptional antiterminator for the sacP-sacA-ywdA operon
| Gene name | sacT |
| Synonyms | ipa-47d |
| Essential | no |
| Product | transcriptional antiterminator |
| Function | regulation of sucrose utilization |
| Interactions involving this protein in SubtInteract: SacT | |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 828 bp, 276 aa |
| Immediate neighbours | ywcJ, ywcI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins
This gene is a member of the following regulons
The SacT regulon: sacP-sacA-ywdA
The gene
Basic information
- Locus tag: BSU38070
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P26212
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- repressed by casamino acids PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP429 (spc), available in Stülke lab
- Expression vector:
- for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Stülke lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Stülke lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Stülke lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Stülke lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Stülke lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Stülke lab
- for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Stülke lab
- for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Stülke lab
- for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
