Difference between revisions of "RnhC"
m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk) |
|||
| Line 96: | Line 96: | ||
* '''Operon:''' ''[[rnhC]]'' {{PubMed|8969504}} | * '''Operon:''' ''[[rnhC]]'' {{PubMed|8969504}} | ||
| − | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnhC_2926031_2926972_1 rnhC] {{PubMed|22383849}} |
| + | |||
| + | * '''Sigma factor:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 102: | Line 104: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 14:48, 16 April 2012
- Description: RNase HIII, endoribonuclease
| Gene name | rnhC |
| Synonyms | ysgB |
| Essential | no |
| Product | Mg2+-dependent RNase HIII |
| Function | endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules |
| MW, pI | 33 kDa, 10.07 |
| Gene length, protein length | 939 bp, 313 aa |
| Immediate neighbours | zapA, pheT |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28620
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
- Protein family: RnhC subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- RnhC interacts with the RNA polymerase PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 2D0B (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), 2D0A (Geobacillus stearothermophilus, 47% identity)
- UniProt: P94541
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197]
[WorldCat.org]
[DOI]
(I p)
Original publications
Additional publications: PubMed
