FolC

• Description: folyl-polyglutamate synthetase
  
  

Gene name	folC
Synonyms
Essential	no
Product	folyl-polyglutamate synthetase
Function	biosynthesis of folate
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Folate
MW, pI	48 kDa, 5.806
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	comC, valS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28080

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1) (according to Swiss-Prot)

• Protein family: folylpolyglutamate synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1FGS (from Lactobacillus casei, 32% identity, 51% similarity) PubMed

• UniProt: Q05865

• KEGG entry: [3]

• E.C. number: 6.3.2.17

Expression and regulation

• Operon: valS-folC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by valine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: riboswitch, transcription termination/antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

D Luo, J Leautey, M Grunberg-Manago, H Putzer
Structure and regulation of expression of the Bacillus subtilis valyl-tRNA synthetase gene.
J Bacteriol: 1997, 179(8);2472-8
[PubMed:9098041] [WorldCat.org] [DOI] (P p)