Difference between revisions of "HemY"
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* '''Locus tag:''' BSU10140 | * '''Locus tag:''' BSU10140 | ||
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+ | [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=hemY_1088197_1089609_1 Expression] | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === |
Revision as of 15:37, 24 January 2012
- Description: protoporphyrinogen IX oxidase
Gene name | hemY |
Synonyms | hemG |
Essential | no |
Product | protoporphyrinogen IX oxidase |
Function | heme biosynthesis |
MW, pI | 51 kDa, 8.042 |
Gene length, protein length | 1410 bp, 470 aa |
Immediate neighbours | hemH, yhgD |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10140
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2 (according to Swiss-Prot)
- Protein family: protoporphyrinogen oxidase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 3I6D
- UniProt: P32397
- KEGG entry: [3]
- E.C. number: 1.3.3.4
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Xiaohong Qin, Lu Sun, Xin Wen, Xue Yang, Ying Tan, Hao Jin, Qiongyao Cao, Weihong Zhou, Zhen Xi, Yuequan Shen
Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis.
J Struct Biol: 2010, 170(1);76-82
[PubMed:19944166]
[WorldCat.org]
[DOI]
(I p)
Lu Sun, Xin Wen, Ying Tan, Heyang Li, Xing Yang, Yuefang Zhao, Baifan Wang, Qiongyao Cao, Congwei Niu, Zhen Xi
Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase.
Amino Acids: 2009, 37(3);523-30
[PubMed:19266155]
[WorldCat.org]
[DOI]
(I p)
M Hansson, M C Gustafsson, C G Kannangara, L Hederstedt
Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity.
Biochim Biophys Acta: 1997, 1340(1);97-104
[PubMed:9217019]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX.
J Bacteriol: 1994, 176(19);5962-70
[PubMed:7928957]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957]
[WorldCat.org]
[DOI]
(P p)