Difference between revisions of "PGP574"
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similar [[plasmid]]: [[pGP172]] | similar [[plasmid]]: [[pGP172]] | ||
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* sequencing primer: | * sequencing primer: | ||
**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’ | **'''T7:''' 5’-TAATACGACTCACTATAGGG -3’ | ||
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| + | |||
| + | <pubmed> 17074746 </pubmed> | ||
Revision as of 08:11, 18 January 2012
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
- sequencing primer:
- T7: 5’-TAATACGACTCACTATAGGG -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Oliver Schilling, Christina Herzberg, Tina Hertrich, Hanna Vörsmann, Dirk Jessen, Sebastian Hübner, Fritz Titgemeyer, Jörg Stülke
Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.
Nucleic Acids Res: 2006, 34(21);6102-15
[PubMed:17074746]
[WorldCat.org]
[DOI]
(I p)
