Difference between revisions of "BsrG"

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−
* '''Description:''' small RNA <br/><br/>
+
* '''Description:''' hydrophobic toxin <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || small RNA
+
|style="background:#ABCDEF;" align="center"| '''Product''' || toxin
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' ||unknown
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length''' ||  bp
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' ||   
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yolA]]'', ''[[yokL]]''
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 38 aa
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yolA]]'', ''[[SR4]]''
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein <br/>'''
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:bsrG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:bsrG_context.gif]]
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<br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/>
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
−
{{SubtiWiki category|[[ncRNA]]}}
+
{{SubtiWiki category|[[toxins, antitoxins and immunity against toxins]]}},
 +
{{SubtiWiki category|[[SPß prophage]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 50: Line 54:
 
* '''SubtiList entry:''' no entry
 
* '''SubtiList entry:''' no entry
 
   
 
   
−
* '''Structure:'''  
+
=== Additional information===
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
 +
 
 +
* '''Protein family:'''
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''[[SubtInteract|Interactions]]:'''
  
−
=== Additional information===
+
* '''[[Localization]]:'''
 +
** membrane protein (based on the presence of a trans-membrane domain)  {{PubMed|22229825}}
 +
 
 +
=== Database entries ===
 +
 
 +
* '''Structure:'''
 +
 
 +
* '''UniProt:'''
 +
 
 +
* '''KEGG entry:'''
  
 +
* '''E.C. number:'''
  
 +
=== Additional information===
  
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[bsrG]]''  {{PubMed|22229825}}
  
−
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]]  {{PubMed|22229825}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expression increases at the onset of the stationary phase {{PubMed|22229825}}
 +
** the mRNA is destabilized upon interaction with the antitoxin RNA [[SR4]] {{PubMed|22229825}}
 +
** the mRNA is degraded by [[Rnc]], [[Rny]] and [[Rnr]] {{PubMed|22229825}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** the mRNA is degraded by [[Rnc]], [[Rny]] and [[Rnr]], degradation is enhanced at higher temperature {{PubMed|22229825}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
 +
** half-life of the mRNA: 15 min  {{PubMed|22229825}}
  
 
=Biological materials =
 
=Biological materials =
Line 78: Line 123:
  
  
−
=Labs working on this gene/RNA=
+
=Labs working on this gene/protein=
  
−
Kouji Nakamura, Tsukuba, University, Japan
+
[[Sabine Brantl]], Jena, Germany
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 86: Line 131:
 
=References=
 
=References=
  
−
<pubmed>18948176, 20525796</pubmed>
+
<pubmed>18948176, 20525796 22229825</pubmed>
  
−
[[Category:small RNA]]
+
[[Category:Protein-coding genes]]

Revision as of 15:33, 11 January 2012

  • Description: hydrophobic toxin

Gene name bsrG
Synonyms
Essential no
Product toxin
Function unknown
MW, pI
Gene length, protein length 294 bp, 38 aa
Immediate neighbours yolA, SR4
Get the DNA and protein
Genetic context
File:BsrG context.gif
This image was kindly provided by SubtiList








Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, SPß prophage, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: no entry

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • UniProt:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression increases at the onset of the stationary phase PubMed
    • the mRNA is destabilized upon interaction with the antitoxin RNA SR4 PubMed
    • the mRNA is degraded by Rnc, Rny and Rnr PubMed
  • Regulatory mechanism:
    • the mRNA is degraded by Rnc, Rny and Rnr, degradation is enhanced at higher temperature PubMed
  • Additional information:
    • half-life of the mRNA: 15 min PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:


Labs working on this gene/protein

Sabine Brantl, Jena, Germany

Your additional remarks

References

Natalie Jahn, Heike Preis, Christoph Wiedemann, Sabine Brantl
BsrG/SR4 from Bacillus subtilis--the first temperature-dependent type I toxin-antitoxin system.
Mol Microbiol: 2012, 83(3);579-98
[PubMed:22229825] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176] [WorldCat.org] [DOI] (P p)

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