Difference between revisions of "HypR"
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− | * '''Description:''' MarR/DUF24-family transcription regulator <br/><br/> | + | * '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''yfkO''(hypO) in response to disulfide stress <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |''yybR'' | + | |''yybR''(hypR) |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''hypR'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator | + | |style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || positively controls nitroreductase gene hypO (yfkO) in response to disulfide stress (diamide, NaOCl) |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | |||
+ | * '''hypO ohrA double mutant more sensitive to NaOCl stress than ohrA single mutant | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
− | + | * '''hypR autoregulated by disulfide stress | |
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' | + | * '''Protein family:'''MarR/DUF24-family regulator |
− | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):'''YdeP,YkvN |
=== Extended information on the protein === | === Extended information on the protein === | ||
− | * '''Kinetic information:''' | + | * '''Kinetic information:'''Cys14 redox sensing Cys, has lower pKa of 6.36 |
− | * '''Domains:''' | + | * '''Domains:'''5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface |
− | * '''Modification:''' | + | * '''Modification:'''oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
Line 80: | Line 82: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
− | * '''Localization:''' | + | * '''Localization:'''cytoplasmic |
=== Database entries === | === Database entries === | ||
− | * '''Structure:''' | + | * '''Structure:'''tba |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37486 P37486] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P37486 P37486] | ||
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''yybR'' (according to [http://dbtbs.hgc.jp/COG/prom/yybR.html DBTBS]) | + | * '''Operon:''' ''yybR''(hypR)(according to [http://dbtbs.hgc.jp/COG/prom/yybR.html DBTBS]) |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
− | * '''Regulation:''' | + | * '''Regulation:'''activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro |
− | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:'''redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and vivo |
− | * '''Additional information:''' | + | * '''Additional information:'''Cys14 and Cys49' are about 8-9 Angstroem apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha helices of the HypR dimer about 4 Angstroem towards each other |
=Biological materials = | =Biological materials = | ||
Line 127: | Line 129: | ||
=References= | =References= | ||
+ | |||
+ | '''Palm, Gottfried; Chi, Bui Khanh; Waack, Paul; Gronau, Katrin; Becher, Dörte; Albrecht, Dirk; Hinrichs, Winfried; Read, Randy and Antelmann, Haike | ||
+ | Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR | ||
+ | Nucleic Acid Research 2012, in press. | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 10:32, 26 December 2011
- Description: MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene yfkO(hypO) in response to disulfide stress
Gene name | yybR(hypR) |
Synonyms | hypR |
Essential | no |
Product | MarR/DUF24-family transcription regulator HypR |
Function | positively controls nitroreductase gene hypO (yfkO) in response to disulfide stress (diamide, NaOCl) |
MW, pI | 14 kDa, 8.415 |
Gene length, protein length | 375 bp, 125 aa |
Immediate neighbours | cotF, ppaC |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
transcription factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU40540
Phenotypes of a mutant
- hypO ohrA double mutant more sensitive to NaOCl stress than ohrA single mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- hypR autoregulated by disulfide stress
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:MarR/DUF24-family regulator
- Paralogous protein(s):YdeP,YkvN
Extended information on the protein
- Kinetic information:Cys14 redox sensing Cys, has lower pKa of 6.36
- Domains:5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface
- Modification:oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:cytoplasmic
Database entries
- Structure:tba
- UniProt: P37486
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yybR(hypR)(according to DBTBS)
- Regulation:activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro
- Regulatory mechanism:redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and vivo
- Additional information:Cys14 and Cys49' are about 8-9 Angstroem apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha helices of the HypR dimer about 4 Angstroem towards each other
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Haike Antelmann,University of Greifswald, Germany
Your additional remarks
References
Palm, Gottfried; Chi, Bui Khanh; Waack, Paul; Gronau, Katrin; Becher, Dörte; Albrecht, Dirk; Hinrichs, Winfried; Read, Randy and Antelmann, Haike Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR Nucleic Acid Research 2012, in press.