PbuO

• Description: similar to hypoxanthine/guanine permease PbuG
  
  

Gene name	pbuO
Synonyms	ytiP
Essential	no
Product	unknown
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation)
MW, pI	45 kDa, 7.484
Gene length, protein length	1296 bp, 432 aa
Immediate neighbours	ytjP, ythQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

• Locus tag: BSU29990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): PbuG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34978

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbuO PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism:
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)