TrxB

• Description: thioredoxin reductase (NADPH)
  
  

Gene name	trxB
Synonyms	yvcH
Essential	yes PubMed
Product	thioredoxin reductase (NADPH)
Function	keeps thioredoxin in the reduced state
MW, pI	34 kDa, 4.99
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	yvcI, cwlO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

electron transport/ other, essential genes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU34790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Thioredoxin + NADP⁺ = thioredoxin disulfide + NADPH (according to Swiss-Prot)

• Protein family: class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P80880

• KEGG entry: [2]

• E.C. number: 1.8.1.9

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • A ncRNA is predicted between yvcI and trxB PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Michiko M Nakano, Ann Lin, Cole S Zuber, Kate J Newberry, Richard G Brennan, Peter Zuber
Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase alpha subunit.
PLoS One: 2010, 5(1);e8664
[PubMed:20084284] [WorldCat.org] [DOI] (I e)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)