ArgH

• Description: argininosuccinate lyase
  
  

Gene name	argH
Synonyms
Essential	no
Product	argininosuccinate lyase
Function	biosynthesis of arginine
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	51 kDa, 4.859
Gene length, protein length	1383 bp, 461 aa
Immediate neighbours	ytzD, argG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU29440

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine (according to Swiss-Prot)

• Protein family: Argininosuccinate lyase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1TJ7 (from Escherichia coli, 47% identity, 64% similarity) PubMed

• UniProt: O34858

• KEGG entry: [2]

• E.C. number: 4.3.2.1

Expression and regulation

• Operon: argG-argH

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC)

• Regulatory mechanism:
  • AhrC: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)