CitA

• Description: minor citrate synthase
  
  

Gene name	citA
Synonyms	citA1
Essential	no
Product	minor citrate synthase
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	40 kDa, 5.778
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	citR, yhdF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

poorly characterized/ putative enzymes

This gene is a member of the following regulons

CitR regulon

The gene

Basic information

• Locus tag: BSU09440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + H₂O + oxaloacetate = citrate + CoA (according to Swiss-Prot)

• Protein family: citrate synthase family (according to Swiss-Prot)

• Paralogous protein(s): MmgD, CitZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2C6X

• UniProt: P39119

• KEGG entry: [3]

• E.C. number: 2.3.3.1

Expression and regulation

• Operon: citA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism: CitR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP676 (erm), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Jin, A L Sonenshein
Transcriptional regulation of Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4680-90
[PubMed:8045899] [WorldCat.org] [DOI] (P p)

S Jin, A L Sonenshein
Identification of two distinct Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4669-79
[PubMed:8045898] [WorldCat.org] [DOI] (P p)