PriA

• Description: primosomal replication factor Y, part of the replisome
  
  

Gene name	priA
Synonyms	yloJ
Essential	yes PubMed
Product	primosomal replication factor Y
Function	DNA replication initiation
Interactions involving this protein in SubtInteract: PriA
MW, pI	91 kDa, 8.279
Gene length, protein length	2415 bp, 805 aa
Immediate neighbours	yloI, def
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU15710

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: helicase C-terminal domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaD-PriA PubMed, DnaB-PriA PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed

• Localization:

Database entries

• Structure:

• UniProt: P94461

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

François Lecointe, Céline Sérèna, Marion Velten, Audrey Costes, Stephen McGovern, Jean-Christophe Meile, Jeffrey Errington, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks.
EMBO J: 2007, 26(19);4239-51
[PubMed:17853894] [WorldCat.org] [DOI] (P p)

S Marsin, S McGovern, S D Ehrlich, C Bruand, P Polard
Early steps of Bacillus subtilis primosome assembly.
J Biol Chem: 2001, 276(49);45818-25
[PubMed:11585815] [WorldCat.org] [DOI] (P p)

H Masai, J Deneke, Y Furui, T Tanaka, K I Arai
Escherichia coli and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication: involvement of ATPase/helicase activity of PriA for inducible stable DNA replication.
Biochimie: 1999, 81(8-9);847-57
[PubMed:10572298] [WorldCat.org] [DOI] (P p)