RecJ

• Description: single-strand DNA-specific exonuclease
  
  

Gene name	yrvE
Synonyms	recJ
Essential	no
Product	single-strand DNA-specific exonuclease
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	87 kDa, 5.181
Gene length, protein length	2358 bp, 786 aa
Immediate neighbours	apt, yrvD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recJ family (according to Swiss-Prot)

• Paralogous protein(s): YorK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA-YrvE PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32044

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yrvE-apt PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP895 (recJ::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J Bacteriol: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] [DOI] (P p)