XylA

• Description: xylose isomerase
  
  

Gene name	xylA
Synonyms
Essential	no
Product	xylose isomerase
Function	utilization of xylan and xylose
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 5.635
Gene length, protein length	1335 bp, 445 aa
Immediate neighbours	xylR, xylB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, XylR regulon

The gene

Basic information

• Locus tag: BSU17600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-xylose = D-xylulose (according to Swiss-Prot)

• Protein family: xylose isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1A0D (Geobacillus stearothermophilus)

• UniProt: P04788

• KEGG entry: [3]

• E.C. number: 5.3.1.5

Expression and regulation

• Operon: xylA-xylB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by xylose (XylR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • XylR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1151 (del aphA3) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Hillen, Erlangen University, Germany Homepage