ProS

• Description: prolyl-tRNA synthetase
  
  

Gene name	proS
Synonyms
Essential	yes PubMed
Product	prolyl-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	63 kDa, 5.012
Gene length, protein length	1692 bp, 564 aa
Immediate neighbours	rasP, polC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU16570

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro) (according to Swiss-Prot)

• Protein family: ProS type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2I4L (from Rhodopseudomonas palustris, 51% identity, 71% similarity) PubMed

• UniProt: O31755

• KEGG entry: [2]

• E.C. number: 6.1.1.15

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thibaut Crepin, Anna Yaremchuk, Mikhail Tukalo, Stephen Cusack
Structures of two bacterial prolyl-tRNA synthetases with and without a cis-editing domain.
Structure: 2006, 14(10);1511-25
[PubMed:17027500] [WorldCat.org] [DOI] (P p)