Difference between revisions of "YvcK"
| Line 1: | Line 1: | ||
| − | * '''Description:''' essential for growth under gluconeogenetic conditions <br/><br/> | + | * '''Description:''' essential for growth under gluconeogenetic conditions, required for correct localization of [[ponA|PBP1]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown | |style="background:#ABCDEF;" align="center"| '''Product''' || unknown | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || localization of [[ponA|PBP1]] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408 | ||
| Line 32: | Line 32: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[carbon core metabolism]]}} | + | {{SubtiWiki category|[[carbon core metabolism]]}}, {{SubtiWiki category|[[cell shape]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 45: | Line 45: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
** unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}} | ** unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}} | ||
| − | ** filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}} | + | ** filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}} |
=== Database entries === | === Database entries === | ||
| Line 81: | Line 81: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' localized as a helical-like pattern {{PubMed|21320184}} |
=== Database entries === | === Database entries === | ||
| Line 131: | Line 131: | ||
=References= | =References= | ||
| − | <pubmed>9237995 16272399, </pubmed> | + | <pubmed>9237995 16272399, 21320184 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:16, 16 February 2011
- Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
| Gene name | yvcK |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | localization of PBP1 |
| MW, pI | 34 kDa, 5.408 |
| Gene length, protein length | 951 bp, 317 aa |
| Immediate neighbours | yvcL, yvcJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
carbon core metabolism, cell shape
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34760
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0052 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: localized as a helical-like pattern PubMed
Database entries
- Structure:
- UniProt: O06974
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
Elodie Foulquier, Frédérique Pompeo, Alain Bernadac, Leon Espinosa, Anne Galinier
The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.
Mol Microbiol: 2011, 80(2);309-18
[PubMed:21320184]
[WorldCat.org]
[DOI]
(I p)
Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399]
[WorldCat.org]
[DOI]
(P p)
A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995]
[WorldCat.org]
[DOI]
(P p)
