ComEB

• Description: late competence protein required for DNA binding and uptake
  
  

Gene name	comEB
Synonyms	comD
Essential	no
Product	late competence protein
Function	genetic compentence
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Protein secretion
MW, pI	20 kDa, 5.368
Gene length, protein length	567 bp, 189 aa
Immediate neighbours	comEC, comEA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon, ComN regulon

The gene

Basic information

• Locus tag: BSU25580

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • localizes to one or both cell poles PubMed

Database entries

• Structure:

• UniProt: P32393

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comEA-comEB-comEC PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • post-translational control by ComN PubMed
  • yutB is required for transcription PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

J Hahn, G Inamine, Y Kozlov, D Dubnau
Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.
Mol Microbiol: 1993, 10(1);99-111
[PubMed:7968523] [WorldCat.org] [DOI] (P p)