Difference between revisions of "SubtInteract"

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'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
 
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
  
==Vizualisation of protein-protein interactions in ''B. subtilis''==
+
==Visualization of protein-protein interactions in ''B. subtilis''==
 
* the beta  version of an [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH interactive protein-protein interaction map] (just replace "PtsH" in the browser line by the protein of your interest)
 
* the beta  version of an [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH interactive protein-protein interaction map] (just replace "PtsH" in the browser line by the protein of your interest)
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]  
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]  
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* [[transcription]]: [[RNA polymerase]]
 
* [[transcription]]: [[RNA polymerase]]
 
* [[translation]]: the [[ribosomal proteins|ribosome]]
 
* [[translation]]: the [[ribosomal proteins|ribosome]]
 +
* [[RNases|RNA processing and degradation]]: the [[RNA degradosome]]
  
 
* [[cell division]]: the [[divisome]]
 
* [[cell division]]: the [[divisome]]

Revision as of 06:45, 4 January 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Visualization of protein-protein interactions in B. subtilis

Protein complexes in B. subtilis

Important publications