SubtInteract

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

• Bacterial Two Hybrid System: BACTH PubMed

• Yeast Two Hybrid System PubMed

• SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed

• TAP-Tag purification PubMed

• in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Vizualisation of protein-protein interactions in B. subtilis

• the beta version of an interactive protein-protein interaction map (just replace "PtsH" in the browser line by the protein of your interest)
• the beta version of SubtInteract

Protein complexes in B. subtilis

• DNA replication: the replisome
• transcription: RNA polymerase
• translation: the ribosome

• cell division: the divisome

• metabolism: the metabolons of glycolysis and the TCA cycle PubMed