Difference between revisions of "SubtInteract"

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'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
 
'''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|20805792,19060903}}
  
==Visualisation of protein-protein interactions in ''B. subtilis''==
+
==Vizualisation of protein-protein interactions in ''B. subtilis''==
 
* the beta  version of an interactive protein-protein interaction map [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH here] (just replace "PtsH" in the browser line by the protein of your interest)
 
* the beta  version of an interactive protein-protein interaction map [http://cellpublisher.gobics.de/subtinteract/interactionList/PtsH here] (just replace "PtsH" in the browser line by the protein of your interest)
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]  
 
* the beta version of [http://subtiwiki.uni-goettingen.de/pathways/SubtInteract/demo_mitlinks/ SubtInteract]  
  
 +
==Protein complexes in ''B. subtilis''==
 +
* [[DNA replication]]: the [[replisome]]
 +
* [[transcription]]: [[RNA polymerase]]
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* [[translation]]: the [[ribosome]]
  
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* [[cell division]]: the [[divisome]]
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 +
* [[metabolism]]: the metabolons of glycolysis and the TCA cycle {{PubMed|19193632,20933603}}
 
==Important publications==
 
==Important publications==
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  </pubmed>
 
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 18366733 10694888 2200717 2441660  </pubmed>

Revision as of 21:43, 3 January 2011

Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.

Methods to detect protein-protein interactions

  • Yeast Two Hybrid System PubMed
  • SPINE: A method to detect in vivo protein-protein interactions after cross-linking PubMed
  • in vivo detection of protein-protein interaction using DivIVA and GFP PubMed

Attention: Each technique detects only about 33% of all interactions PubMed

Vizualisation of protein-protein interactions in B. subtilis

  • the beta version of an interactive protein-protein interaction map here (just replace "PtsH" in the browser line by the protein of your interest)
  • the beta version of SubtInteract

Protein complexes in B. subtilis

Important publications