YvcK

• Description: essential for growth under gluconeogenetic conditions
  
  

Gene name	yvcK
Synonyms
Essential	no
Product	unknown
Function	unknown
MW, pI	34 kDa, 5.408
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yvcL, yvcJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34760

Phenotypes of a mutant

• • unable to grow with gluconeogenic substrates as single carbon source PubMed
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

carbon core metabolism

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0052 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O06974

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

References

Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399] [WorldCat.org] [DOI] (P p)

A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995] [WorldCat.org] [DOI] (P p)