LipB

• Description: extracellular esterase, lipase
  
  

Gene name	lipB
Synonyms	yfiP
Essential	no
Product	extracellular esterase, lipase
Function	lipid degradation
MW, pI	22 kDa, 9.677
Gene length, protein length	630 bp, 210 aa
Immediate neighbours	padR, yfiQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of lipids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): Lip

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q79F14

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lipB (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thorsten Eggert, Ulf Brockmeier, Melloney J Dröge, Wim J Quax, Karl-Erich Jaeger
Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH.
FEMS Microbiol Lett: 2003, 225(2);319-24
[PubMed:12951259] [WorldCat.org] [DOI] (P p)

T Eggert, G van Pouderoyen, B W Dijkstra, K E Jaeger
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure.
FEBS Lett: 2001, 502(3);89-92
[PubMed:11583117] [WorldCat.org] [DOI] (P p)

T Eggert, G Pencreac'h, I Douchet, R Verger, K E Jaeger
A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase.
Eur J Biochem: 2000, 267(21);6459-69
[PubMed:11029590] [WorldCat.org] [DOI] (P p)

G Kobayashi, J Toida, T Akamatsu, H Yamamoto, T Shida, J Sekiguchi
Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants.
FEMS Microbiol Lett: 2000, 188(2);165-9
[PubMed:10913700] [WorldCat.org] [DOI] (P p)