HutG

• Description: formiminoglutamate hydrolase
  
  

Gene name	hutG
Synonyms
Essential	no
Product	formiminoglutamate hydrolase
Function	histidine utilization
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	34 kDa, 5.439
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	hutI, hutM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39380

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-formimidoyl-L-glutamate + H₂O = L-glutamate + formamide (according to Swiss-Prot)

• Protein family: arginase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42068

• KEGG entry: [3]

• E.C. number: 3.5.3.8

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by histidine (HutP) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed
  • HutP: transcriptional antitermination at a protein-dependent RNA switch located between hutP and hutH PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

J M Zalieckas, L V Wray, S H Fisher
trans-acting factors affecting carbon catabolite repression of the hut operon in Bacillus subtilis.
J Bacteriol: 1999, 181(9);2883-8
[PubMed:10217782] [WorldCat.org] [DOI] (P p)

S H Fisher, K Rohrer, A E Ferson
Role of CodY in regulation of the Bacillus subtilis hut operon.
J Bacteriol: 1996, 178(13);3779-84
[PubMed:8682780] [WorldCat.org] [DOI] (P p)

L V Wray, S H Fisher
Analysis of Bacillus subtilis hut operon expression indicates that histidine-dependent induction is mediated primarily by transcriptional antitermination and that amino acid repression is mediated by two mechanisms: regulation of transcription initiation and inhibition of histidine transport.
J Bacteriol: 1994, 176(17);5466-73
[PubMed:8071225] [WorldCat.org] [DOI] (P p)