CitG

• Description: fumarase
  
  

Gene name	citG
Synonyms
Essential	no
Product	fumarate hydratase
Function	TCA cycle
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	50 kDa, 5.475
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	yuxN, gerAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

carbon core metabolism

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate = fumarate + H₂O (according to Swiss-Prot)

• Protein family: Fumarase subfamily (according to Swiss-Prot)

• Paralogous protein(s): AnsB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Mdh-CitG PubMed
  • CitG-CitB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1YFE (E.coli)

• UniProt: P07343

• KEGG entry: [3]

• E.C. number: 4.2.1.2

Expression and regulation

• Operon: citG PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation: constitutive

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP718 (spec), available in Stülke lab

• Expression vector:
  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)

• lacZ fusion: pGP387 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Frederik M Meyer, Jan Gerwig, Elke Hammer, Christina Herzberg, Fabian M Commichau, Uwe Völker, Jörg Stülke
Physical interactions between tricarboxylic acid cycle enzymes in Bacillus subtilis: evidence for a metabolon.
Metab Eng: 2011, 13(1);18-27
[PubMed:20933603] [WorldCat.org] [DOI] (I p)

V A Price, I M Feavers, A Moir
Role of sigma H in expression of the fumarase gene (citG) in vegetative cells of Bacillus subtilis 168.
J Bacteriol: 1989, 171(11);5933-9
[PubMed:2509423] [WorldCat.org] [DOI] (P p)

K M Tatti, H L Carter, A Moir, C P Moran
Sigma H-directed transcription of citG in Bacillus subtilis.
J Bacteriol: 1989, 171(11);5928-32
[PubMed:2509422] [WorldCat.org] [DOI] (P p)

I M Feavers, V Price, A Moir
The regulation of the fumarase (citG) gene of Bacillus subtilis 168.
Mol Gen Genet: 1988, 211(3);465-71
[PubMed:3130545] [WorldCat.org] [DOI] (P p)

J S Miles, J R Guest
Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
Nucleic Acids Res: 1985, 13(1);131-40
[PubMed:3923430] [WorldCat.org] [DOI] (P p)