CheB

• Description: MCP-glutamate methylesterase / two-component response regulator-like
  
  

Gene name	cheB
Synonyms	cheL
Essential	no
Product	MCP-glutamate methylesterase / two-component response regulator-like
Function	motility, chemotaxis
MW, pI	38 kDa, 8.119
Gene length, protein length	1071 bp, 357 aa
Immediate neighbours	ylxH, cheA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

motility and chemotaxis

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H₂O = protein L-glutamate + methanol (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q05522

• KEGG entry: [3]

• E.C. number: 3.1.1.61

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: