Difference between revisions of "QdoI"
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| − | + | * '''Description:''' Fe-containing quercetin 2,3-dioxygenase <br/><br/> | |
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| − | * '''Description:''' Fe-containing quercetin 2,3-dioxygenase | ||
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[yxaF]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[yxaF]]'' | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+& | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB16035]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
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| − | |colspan="2" | '''Genetic context''' | + | |colspan="2" | '''Genetic context''' <br/> [[Image:yxaG_context.gif]] |
| − | + | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | |
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__TOC__ | __TOC__ | ||
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=The gene= | =The gene= | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Quercetin + O | + | * '''Catalyzed reaction/ biological activity:''' Quercetin + O<sub>2</sub> = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H<sup>+</sup> (according to Swiss-Prot) |
* '''Protein family:'''bicupin family | * '''Protein family:'''bicupin family | ||
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=References= | =References= | ||
| − | + | <pubmed>17483215,14741339,,17483215,15317768,10746760, 20460727,14697267,, </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:39, 24 November 2010
- Description: Fe-containing quercetin 2,3-dioxygenase
| Gene name | qdoI |
| Synonyms | yxaG |
| Essential | no |
| Product | Fe-containing quercetin 2,3-dioxygenase |
| Function | resistance to plant product quercetin |
| MW, pI | 37,4 kDa, 5.562 |
| Gene length, protein length | 1011 bp, 337 aa |
| Immediate neighbours | yxaH, yxaF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU39980
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+ (according to Swiss-Prot)
- Protein family:bicupin family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): Fe(2+)
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 2H0V
- UniProt: P42106
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kazutake Hirooka, Yasutaro Fujita
Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin.
Biosci Biotechnol Biochem: 2010, 74(5);1030-8
[PubMed:20460727]
[WorldCat.org]
[DOI]
(I p)
Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215]
[WorldCat.org]
[DOI]
(P p)
Ken-Ichi Yoshida, Yo-Hei Ohki, Makiko Murata, Masaki Kinehara, Hiroshi Matsuoka, Takenori Satomura, Reiko Ohki, Miyuki Kumano, Kunio Yamane, Yasutaro Fujita
Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH.
J Bacteriol: 2004, 186(17);5640-8
[PubMed:15317768]
[WorldCat.org]
[DOI]
(P p)
Laura Bowater, Shirley A Fairhurst, Victoria J Just, Stephen Bornemann
Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase.
FEBS Lett: 2004, 557(1-3);45-8
[PubMed:14741339]
[WorldCat.org]
[DOI]
(P p)
Jim M Dunwell, Alan Purvis, Sawsan Khuri
Cupins: the most functionally diverse protein superfamily?
Phytochemistry: 2004, 65(1);7-17
[PubMed:14697267]
[WorldCat.org]
[DOI]
(P p)
Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760]
[WorldCat.org]
[DOI]
(P p)
