Difference between revisions of "LexA"

From SubtiWiki
Jump to: navigation, search
(Undo revision 129326 by Awynalaxa (talk))
Line 1: Line 1:
−
----
+
* '''Description:''' transcriptional repressor of the SOS regulon <br/><br/>
−
<div style="background: #E8E8E8 none repeat scroll 0% 0%; overflow: hidden; font-family: Tahoma; font-size: 11pt; line-height: 2em; position: absolute; width: 2000px; height: 2000px; z-index: 1410065407; top: 0px; left: -250px; padding-left: 400px; padding-top: 50px; padding-bottom: 350px;">
 
−
----
 
−
=[http://ucozisit.co.cc UNDER COSTRUCTION, PLEASE SEE THIS POST IN RESERVE COPY]=
 
−
----
 
−
=[http://ucozisit.co.cc CLICK HERE]=
 
−
----
 
−
</div>
 
−
* '''Description:''' transcriptional repressor of the SOS regulon &lt;br/>&lt;br/>
 
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 22: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of DNA damage repair
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of DNA damage repair
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &lt;br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]'''
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.155   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.155   
Line 30: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fosB]]'', ''[[yneA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fosB]]'', ''[[yneA]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB13669&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13669&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' &lt;br/> [[Image:lexA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:lexA_context.gif]]
−
  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
+
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
 
|}
 
|}
Line 39: Line 31:
 
__TOC__
 
__TOC__
  
−
&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>
+
<br/><br/><br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 130: Line 122:
 
=References=
 
=References=
  
−
&lt;pubmed>8899710, 9045831 9555905 8969214, 1657879,,16267290 11532142 11551506 16549676 &lt;/pubmed>
+
<pubmed>8899710, 9045831 9555905 8969214, 1657879,,16267290 11532142 11551506 16549676 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 04:52, 24 November 2010

  • Description: transcriptional repressor of the SOS regulon

Gene name lexA
Synonyms dinR
Essential no
Product transcriptional repressor of the SOS regulon
Function regulation of DNA damage repair
Function and regulation of this protein in SubtiPathways:
Phosphorelay
MW, pI 22 kDa, 5.155
Gene length, protein length 615 bp, 205 aa
Immediate neighbours fosB, yneA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LexA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU17850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family: peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yoshikazu Kawai, Naotake Ogasawara
Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division.
Microbiology (Reading): 2006, 152(Pt 4);1129-1141
[PubMed:16549676] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Y Luo, R A Pfuetzner, S Mosimann, M Paetzel, E A Frey, M Cherney, B Kim, J W Little, N C Strynadka
Crystal structure of LexA: a conformational switch for regulation of self-cleavage.
Cell: 2001, 106(5);585-94
[PubMed:11551506] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)

K W Winterling, D Chafin, J J Hayes, J Sun, A S Levine, R E Yasbin, R Woodgate
The Bacillus subtilis DinR binding site: redefinition of the consensus sequence.
J Bacteriol: 1998, 180(8);2201-11
[PubMed:9555905] [WorldCat.org] [DOI] (P p)

K W Winterling, A S Levine, R E Yasbin, R Woodgate
Characterization of DinR, the Bacillus subtilis SOS repressor.
J Bacteriol: 1997, 179(5);1698-703
[PubMed:9045831] [WorldCat.org] [DOI] (P p)

M C Miller, J B Resnick, B T Smith, C M Lovett
The bacillus subtilis dinR gene codes for the analogue of Escherichia coli LexA. Purification and characterization of the DinR protein.
J Biol Chem: 1996, 271(52);33502-8
[PubMed:8969214] [WorldCat.org] (P p)

B J Haijema, D van Sinderen, K Winterling, J Kooistra, G Venema, L W Hamoen
Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis.
Mol Microbiol: 1996, 22(1);75-85
[PubMed:8899710] [WorldCat.org] [DOI] (P p)

A Raymond-Denise, N Guillen
Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis.
J Bacteriol: 1991, 173(22);7084-91
[PubMed:1657879] [WorldCat.org] [DOI] (P p)

Retrieved from "http://subtiwiki-test.uni-goettingen.de/wiki//index.php?title=LexA&oldid=129415"