Difference between revisions of "YngI"
Line 1: | Line 1: | ||
− | * '''Description:''' aceto- | + | * '''Description:''' aceto-acetyl-CoA ligase, may be involved in surfactin production <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || aceto- | + | |style="background:#ABCDEF;" align="center"| '''Product''' || aceto-acetyl-CoA ligase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization | ||
Line 56: | Line 56: | ||
* '''Protein family:''' | * '''Protein family:''' | ||
− | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' [[LcfA]], [[LcfB]], [[YhfT]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
Line 101: | Line 101: | ||
=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' | + | * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}} |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Line 118: | Line 118: | ||
=References= | =References= | ||
− | <pubmed>15699190,12662922 19935659 </pubmed> | + | <pubmed>15699190,12662922 19935659 20797616</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:21, 1 September 2010
- Description: aceto-acetyl-CoA ligase, may be involved in surfactin production
Gene name | yngI |
Synonyms | |
Essential | no |
Product | aceto-acetyl-CoA ligase |
Function | mother cell metabolism, leucine utilization |
MW, pI | 61 kDa, 5.978 |
Gene length, protein length | 1647 bp, 549 aa |
Immediate neighbours | yngH, yngJ |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed
- Protein family:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O31826
- KEGG entry: [3]
- E.C. number: 6.2.1.16
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: available in Mohamed Marahiel's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616]
[WorldCat.org]
[DOI]
(I p)
Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659]
[WorldCat.org]
[DOI]
(I p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922]
[WorldCat.org]
[DOI]
(P p)