SpoIIIC

• Description: RNA polymerase sporulation-specific sigma factor (SigK) (C-terminal half), other part: spoIVCB
  
  

Gene name	spoIIIC
Synonyms	sigK, spoIVD, spoIVE
Essential	no
Product	RNA polymerase sporulation-specific sigma factor (SigK) (C-terminal half)
Function	late mother cell-specific gene expression
MW, pI	16 kDa, 9.608
Gene length, protein length	414 bp, 138 aa
Immediate neighbours	yqaB, yrkS
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU26390

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigK-(RpoB-RpoC)

• Localization:

Database entries

• Structure:

• UniProt: P12254

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigK (composed of spoIVCB-spoIIIC) PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed
  • SpoIIID: transcription activation PubMed

• Additional information:
  • SigK is activated by processing of membrane-bound pro-SigK by the SpoIVFB metalloprotease PubMed
  • spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: