Difference between revisions of "PGP172"
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[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']] | [[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']] | ||
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| − | + | vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein | |
| − | + | host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | |
| + | |||
| + | constructed in the [[Stülke]] lab | ||
| + | |||
| + | in E. coli: ampicillin resistance | ||
| + | |||
| + | based on pET3C | ||
| + | |||
| + | similar [[plasmid]]: [[pGP574]] | ||
| + | |||
| + | sequencing primer: | ||
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | *'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | ||
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | *'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
<pubmed> 14717704 </pubmed> | <pubmed> 14717704 </pubmed> | ||
Revision as of 10:18, 14 April 2010
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704]
[WorldCat.org]
[DOI]
(P p)
