Difference between revisions of "RapF"

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=Expression and regulation=
 
=Expression and regulation=
 
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* '''Operon:''' ''[[rapF]]-[[phrF]]'' {{PubMed|11466295}}
* '''Operon:'''  
 
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' repressed by glucose (5-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] 
+
* '''Regulation:''' repressed by glucose (5-fold) ([[CcpA]]) {{PubMed|12850135}}
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
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* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression {{PubMed|12850135}}
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** [[ComA]]: transcription activation {{PubMed|15968044}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=References=
 
=References=
  
<pubmed>12850135, 15968044</pubmed>
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<pubmed>12850135, 15968044 11466295 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:04, 3 February 2010

Gene name rapF
Synonyms ywhJ
Essential no
Product response regulator aspartate phosphatase
Function control of ComA activity
MW, pI 45 kDa, 5.18
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours ywhK, phrF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RapF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: control of ComA activity PubMed
  • Protein family: RAP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: binding of PhrF to RapF prevents it from binding ComA PubMed
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (5-fold) (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)