Difference between revisions of "NarK"

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(Expression and regulation)
(Expression and regulation)
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* '''Regulation:'''  
 
* '''Regulation:'''  
** ''[[narK]]'': expressed under anaerobic conditions ([[Fnr]]) {{PubMed|8846791,16428414}}
+
** expressed under anaerobic conditions ([[Fnr]]) {{PubMed|8846791,16428414}}
 
** repressed unless NO is present under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
 
** repressed unless NO is present under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
  

Revision as of 19:47, 3 February 2010

  • Description: nitrite extrusion protein

Gene name narK
Synonyms
Essential no
Product nitrite extrusion protein
Function nitrate respiration, nitrite export
MW, pI 42 kDa, 10.099
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours fnr, argS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NarK context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU37320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): NasA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (Fnr) PubMed
    • repressed unless NO is present under anaerobic conditions (NsrR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)