Difference between revisions of "NarJ"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
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* '''Operon:''' ''[[narG]]-[[narH]]-[[narJ]]-[[narI]]'' {{PubMed|8846791,16428414}}
  
* '''Operon:'''
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8846791,16428414}}
 
 
* '''[[Sigma factor]]:'''  
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed under anaerobic conditions ([[Fnr]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8846791,16428414 PubMed]
+
** expressed under anaerobic conditions ([[Fnr]]) {{PubMed|8846791,16428414}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[Fnr]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8846791,16428414 PubMed]
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** [[Fnr]]: transcription activation {{PubMed|8846791,16428414}}
  
* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 16:42, 28 January 2010

  • Description: nitrate reductase (protein J)

Gene name narJ
Synonyms
Essential no
Product nitrate reductase (protein J)
Function nitrate respiration, nitrogen assimilation
MW, pI 21 kDa, 4.615
Gene length, protein length 552 bp, 184 aa
Immediate neighbours narI, narH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NarJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (Fnr) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)