Difference between revisions of "PucM"
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=Expression and regulation= | =Expression and regulation= | ||
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* '''Operon:''' | * '''Operon:''' | ||
| + | ** ''[[pucR]]-[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12823818}} | ||
| + | ** ''[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12029039}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| + | ** ''[[pucJ]]'': [[SigA]] {{PubMed|12029039}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) | + | ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}} |
| − | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) | + | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[TnrA]]: transcription activation | + | ** [[TnrA]]: transcription activation {{PubMed|12823818}} |
| − | ** [PucR]]: transcription activation | + | ** [[PucR]]: transcription activation {{PubMed|12029039}} |
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 15:26, 28 December 2009
- Description: uricase
| Gene name | pucM |
| Synonyms | yunM |
| Essential | no |
| Product | uricase |
| Function | purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 13 kDa, 5.082 |
| Gene length, protein length | 363 bp, 121 aa |
| Immediate neighbours | pucL, pucE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU32460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)
- Protein family: 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 2H0E
- UniProt: O32142
- KEGG entry: [3]
- E.C. number: 3.5.2.17
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
