Difference between revisions of "PucK"
(→Expression and regulation) |
|||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | |||
* '''Operon:''' | * '''Operon:''' | ||
| + | ** ''[[pucR]]-[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12823818}} | ||
| + | ** ''[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12029039}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| + | ** ''[[pucJ]]'': [[SigA]] {{PubMed|12029039}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) | + | ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}} |
| − | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) | + | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[TnrA]]: transcription activation | + | ** [[TnrA]]: transcription activation {{PubMed|12823818}} |
| − | ** [PucR]]: transcription activation | + | ** [[PucR]]: transcription activation {{PubMed|12029039}} |
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 15:24, 28 December 2009
- Description: uric acid permease
| Gene name | pucK |
| Synonyms | yunK |
| Essential | no |
| Product | uric acid permease |
| Function | purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 44 kDa, 8.348 |
| Gene length, protein length | 1290 bp, 430 aa |
| Immediate neighbours | pucJ, pucL |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU32440
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: xanthine/uracil permease family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32140
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818]
[WorldCat.org]
[DOI]
(P p)
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
