AlaS

• Description: alanine-tRNA synthetase
  
  

Gene name	alaS
Synonyms
Essential	yes PubMed
Product	alanine-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	97 kDa, 4.922
Gene length, protein length	2634 bp, 878 aa
Immediate neighbours	yrzL, yrrI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27410

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YFS (enzyme from Aquifex aeolicus) PubMed

• UniProt: O34526

• KEGG entry: [2]

• E.C. number: 6.1.1.7

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • induced by alanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Manal A Swairjo, Paul R Schimmel
Breaking sieve for steric exclusion of a noncognate amino acid from active site of a tRNA synthetase.
Proc Natl Acad Sci U S A: 2005, 102(4);988-93
[PubMed:15657145] [WorldCat.org] [DOI] (P p)