Difference between revisions of "Obg"
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa | ||
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− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||'' [[pheB]]'', ''[[spo0B]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14752]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14752]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
Revision as of 09:12, 7 December 2009
- Description: GTP-binding protein involved in initiation of sporulation
Gene name | obg |
Synonyms | |
Essential | Yes |
Product | GTP-binding protein |
Function | may be required to stimulate activity of the phosphorelay that activates Spo0A |
MW, pI | 47 kDa, 4.859 |
Gene length, protein length | 1284 bp, 428 aa |
Immediate neighbours | pheB, spo0B |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU27920
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: GTP1/OBG family (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
- Modification:
- Cofactor(s):
GDP, GTP and probably ppGpp
- Effectors of protein activity:
- Interactions:
Interaction with Rsb proteins.
- Localization:
Database entries
- UniProt: P20964
- KEGG entry: [3]
- E.C. number:
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
Expression and regulation
- Sigma factor: SigA
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Bill Haldenwang, San Antonio, USA
- Naotake Ogasawara, Nara, Japan
Your additional remarks
References
Shrin Kuo, Borries Demeler, W G Haldenwang
The growth-promoting and stress response activities of the Bacillus subtilis GTP binding protein Obg are separable by mutation.
J Bacteriol: 2008, 190(20);6625-35
[PubMed:18689482]
[WorldCat.org]
[DOI]
(I p)
Shrin Kuo, Shuyu Zhang, Robyn L Woodbury, W G Haldenwang
Associations between Bacillus subtilis sigmaB regulators in cell extracts.
Microbiology (Reading): 2004, 150(Pt 12);4125-36
[PubMed:15583165]
[WorldCat.org]
[DOI]
(P p)
Shuyu Zhang, W G Haldenwang
Guanine nucleotides stabilize the binding of Bacillus subtilis Obg to ribosomes.
Biochem Biophys Res Commun: 2004, 322(2);565-9
[PubMed:15325267]
[WorldCat.org]
[DOI]
(P p)
John Buglino, Vincent Shen, Payam Hakimian, Christopher D Lima
Structural and biochemical analysis of the Obg GTP binding protein.
Structure: 2002, 10(11);1581-92
[PubMed:12429099]
[WorldCat.org]
[DOI]
(P p)
Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945]
[WorldCat.org]
[DOI]
(P p)
J M Scott, J Ju, T Mitchell, W G Haldenwang
The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes.
J Bacteriol: 2000, 182(10);2771-7
[PubMed:10781545]
[WorldCat.org]
[DOI]
(P p)
J M Scott, W G Haldenwang
Obg, an essential GTP binding protein of Bacillus subtilis, is necessary for stress activation of transcription factor sigma(B).
J Bacteriol: 1999, 181(15);4653-60
[PubMed:10419966]
[WorldCat.org]
[DOI]
(P p)
K M Welsh, K A Trach, C Folger, J A Hoch
Biochemical characterization of the essential GTP-binding protein Obg of Bacillus subtilis.
J Bacteriol: 1994, 176(23);7161-8
[PubMed:7961487]
[WorldCat.org]
[DOI]
(P p)
J Kok, K A Trach, J A Hoch
Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg.
J Bacteriol: 1994, 176(23);7155-60
[PubMed:7961486]
[WorldCat.org]
[DOI]
(P p)
K Trach, J A Hoch
The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.
J Bacteriol: 1989, 171(3);1362-71
[PubMed:2537815]
[WorldCat.org]
[DOI]
(P p)