NifS

• Description: cysteine desulfurase, required for NAD biosynthesis
  
  

Gene name	nifS
Synonyms	iscS
Essential	no
Product	cysteine desulfurase
Function	NAD biosynthesis
MW, pI	43 kDa, 6.425
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	nadB, nadR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

• Protein family: NifS/iscS subfamily (according to Swiss-Prot)

• Paralogous protein(s): YrvO, NifZ, Csd

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P38033

• KEGG entry: [3]

• E.C. number: 2.8.1.7

Expression and regulation

• Operon: nifS-nadR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.
J Bacteriol: 1993, 175(5);1423-32
[PubMed:8444804] [WorldCat.org] [DOI] (P p)