Difference between revisions of "SpoIIIC"
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| − | * '''Description:''' RNA polymerase sporulation-specific sigma factor ( | + | * '''Description:''' RNA polymerase sporulation-specific sigma factor ([[SigK]]) (C-terminal half), other part: ''[[spoIVCB]]''<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sporulation-specific sigma factor <br/>( | + | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sporulation-specific sigma factor <br/>([[SigK]]) (C-terminal half) |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || late mother cell-specific gene expression | |style="background:#ABCDEF;" align="center"|'''Function''' || late mother cell-specific gene expression | ||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[sigK]]'' (composed of ''[[spoIVCB]]-[[spoIIIC]]'') {{PubMed|2536191}} |
| − | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed| | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,2841290}}, [[SigK]] {{PubMed|2841290}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]], [[SpoIIID]]) {{PubMed|15699190,2841290,10075739,7966271}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[GerE]]: transcription repression {{PubMed|10075739}} | ||
| + | ** [[SpoIIID]]: transcription activation {{PubMed|7966271}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** [[SigK]] is activated by processing of membrane-bound pro-[[SigK]] by the [[SpoIVFB]] metalloprotease {{PubMed|19805276}} | ||
| + | ** ''spoIVCB'' and ''[[spoIIIC]]'' form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence. | ||
=Biological materials = | =Biological materials = | ||
Revision as of 19:59, 24 November 2009
- Description: RNA polymerase sporulation-specific sigma factor (SigK) (C-terminal half), other part: spoIVCB
| Gene name | spoIIIC |
| Synonyms | sigK, spoIVD, spoIVE |
| Essential | no |
| Product | RNA polymerase sporulation-specific sigma factor (SigK) (C-terminal half) |
| Function | late mother cell-specific gene expression |
| MW, pI | 16 kDa, 9.608 |
| Gene length, protein length | 414 bp, 138 aa |
| Immediate neighbours | yqaB, yrkS |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU26390
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P12254
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
B Kunkel, R Losick, P Stragier
The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.
Genes Dev: 1990, 4(4);525-35
[PubMed:2163341]
[WorldCat.org]
[DOI]
(P p)
P Stragier, B Kunkel, L Kroos, R Losick
Chromosomal rearrangement generating a composite gene for a developmental transcription factor.
Science: 1989, 243(4890);507-12
[PubMed:2536191]
[WorldCat.org]
[DOI]
(P p)
J Errington, S Rong, M S Rosenkrantz, A L Sonenshein
Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC.
J Bacteriol: 1988, 170(3);1162-7
[PubMed:3125151]
[WorldCat.org]
[DOI]
(P p)
J Errington
Two separable functional domains in the sigma-subunit of RNA polymerase in Bacillus subtilis?
FEBS Lett: 1987, 224(2);257-60
[PubMed:3121385]
[WorldCat.org]
[DOI]
(P p)
S M Turner, J Errington, J Mandelstam
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons.
J Gen Microbiol: 1986, 132(11);2995-3003
[PubMed:3114422]
[WorldCat.org]
[DOI]
(P p)
