Difference between revisions of "Rnz"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
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* '''Operon:''' ''rnz'' (according to [http://dbtbs.hgc.jp/COG/prom/yqjK.html DBTBS])
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 20:37, 18 November 2009

Gene name rnz
Synonyms yqjK
Essential yes PubMed
Product endoribonuclease Z
Function processing of CCA-less tRNA precursors
MW, pI 33 kDa, 5.805
Gene length, protein length 921 bp, 307 aa
Immediate neighbours yqjL, zwf
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqjK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23840

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs (according to Swiss-Prot) processing of CCA-less tRNA precursors
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2FK6 (in complex with tRNA-Thr), 1Y44
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: rnz (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)

Inés Li de la Sierra-Gallay, Nathalie Mathy, Olivier Pellegrini, Ciarán Condon
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA.
Nat Struct Mol Biol: 2006, 13(4);376-7
[PubMed:16518398] [WorldCat.org] [DOI] (P p)

Inés Li de la Sierra-Gallay, Olivier Pellegrini, Ciarán Condon
Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z.
Nature: 2005, 433(7026);657-61
[PubMed:15654328] [WorldCat.org] [DOI] (I p)

Olivier Pellegrini, Jamel Nezzar, Anita Marchfelder, Harald Putzer, Ciarán Condon
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis.
EMBO J: 2003, 22(17);4534-43
[PubMed:12941704] [WorldCat.org] [DOI] (P p)