Difference between revisions of "Tpi"
(→Expression and regulation) |
|||
| Line 93: | Line 93: | ||
* '''Operon:''' | * '''Operon:''' | ||
| − | ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' | + | ** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}} |
| − | ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' | + | ** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}} |
| − | * '''Sigma factor:''' [[SigA]] | + | * '''Sigma factor:''' [[SigA]] {{PubMed|11489127}} |
| − | * '''Regulation:''' expression activated by glucose ( | + | * '''Regulation:''' |
| − | **''[[cggR]]'': | + | ** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] |
| − | **''[[pgk]]'': constitutive | + | ** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}} |
| + | ** ''[[pgk]]'': constitutive {{PubMed|11489127}} | ||
| − | * '''Regulatory mechanism:''' transcription repression by [[CggR]] | + | * '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}} |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 19:53, 4 October 2009
- Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme
| Gene name | tpi |
| Synonyms | tpiA |
| Essential | yes |
| Product | triosephosphate isomerase |
| Function | enzyme in glycolysis/ gluconeogenesis |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
| MW, pI | 26,9 kDa, 4.79 |
| Gene length, protein length | 759 bp, 253 amino acids |
| Immediate neighbours | pgk, pgm |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU33920
Phenotypes of a mutant
- Essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
- Protein family: triosephosphate isomerase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Ser-213 PubMed
- Cofactor(s):
- Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot), cytoplasm PubMed
Database entries
- Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
- UniProt: P27876
- KEGG entry: [3]
- E.C. number: 5.3.1.1
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant: GP700 (cat), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
