Difference between revisions of "PAC7"
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*'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘ | *'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘ | ||
− | + | <pubmed> 1715859 </pubmed> | |
− |
Revision as of 18:36, 7 September 2009
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: kanamycin resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859]
[WorldCat.org]
[DOI]
(P p)