Difference between revisions of "PAC5"
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[[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | [[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | ||
| − | + | Vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | |
| − | + | Does not replicate in ''B. subtilis'', integrates into the ''amyE'' site | |
| − | + | Constructed in the Rapoport lab | |
| − | in ''E. coli'': ampicillin resistance | + | * in ''E. coli'': ampicillin resistance |
| − | in ''B. subtilis'': chloramphenicol resistance | + | * in ''B. subtilis'': chloramphenicol resistance |
| − | similar vectors: [[pAC6]], [[pAC7]] | + | * similar vectors: [[pAC6]], [[pAC7]] |
| − | sequencing | + | sequencing primers: |
*'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
*'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
*'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | ||
| − | + | <pubmed> 1619665 </pubmed> | |
| − | |||
Revision as of 18:34, 7 September 2009
Vector for the construction of translational lacZ fusions that can be integrated at the amyE site
Does not replicate in B. subtilis, integrates into the amyE site
Constructed in the Rapoport lab
- in E. coli: ampicillin resistance
- in B. subtilis: chloramphenicol resistance
sequencing primers:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence.
J Mol Biol: 1992, 226(1);85-99
[PubMed:1619665]
[WorldCat.org]
[DOI]
(P p)
