Difference between revisions of "YyaF"
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* '''Mutant:''' | * '''Mutant:''' | ||
− | * '''Expression vector:''' | + | * '''Expression vector:''' |
+ | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP836, available in [[Stülke]] lab | ||
+ | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP844, available in [[Stülke]] lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 18:21, 1 September 2009
- Description: GTP-binding protein/ GTPase
Gene name | yyaF |
Synonyms | |
Essential | no |
Product | GTP-binding protein/ GTPase |
Function | unknown |
MW, pI | 39 kDa, 4.659 |
Gene length, protein length | 1098 bp, 366 aa |
Immediate neighbours | rpsF, yyaE |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU40920
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: EngD subfamily (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P37518
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
Your additional remarks
References
Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945]
[WorldCat.org]
[DOI]
(P p)