Difference between revisions of "YfkN"
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed] | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || 2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || 2',3' cyclic nucleotide phosphodiesterase, <br/>2' (or 3') nucleotidase and 5' nucleotidase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || probably phosphate acquisition | |style="background:#ABCDEF;" align="center"|'''Function''' || probably phosphate acquisition | ||
| Line 92: | Line 92: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''yfkN'' {{PubMed|16291680}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' repressed by glucose (5.2-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | + | * '''Regulation:''' |
| − | ** expressed under conditions of phosphate limitation ([[PhoP]]) | + | ** repressed by glucose (5.2-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] |
| + | ** expressed under conditions of phosphate limitation ([[PhoP]]) {{PubMed|16291680}} | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| − | ** [[ | + | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] |
| + | ** [[PhoP]]: transcription activation {{PubMed|16291680}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 18:27, 31 August 2009
- Description: extracellular 2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
| Gene name | yfkN |
| Synonyms | |
| Essential | yes PubMed |
| Product | 2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase |
| Function | probably phosphate acquisition |
| MW, pI | 159 kDa, 5.021 |
| Gene length, protein length | 4386 bp, 1462 aa |
| Immediate neighbours | yfkO, yfkM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU07840
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): YhcR
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure:
- UniProt: O34313
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yfkN PubMed
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Alan C Ward, Anil Wipat, Colin R Harwood
Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8063-80
[PubMed:16291680]
[WorldCat.org]
[DOI]
(P p)
Régis Chambert, Yannick Pereira, Marie-Françoise Petit-Glatron
Purification and characterization of YfkN, a trifunctional nucleotide phosphoesterase secreted by Bacillus subtilis.
J Biochem: 2003, 134(5);655-60
[PubMed:14688230]
[WorldCat.org]
[DOI]
(P p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
